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Human rhinovirus 57

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價格:

編號:B222181

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

Human rhinovirus 57

商品貨號

B222181

Classification

Picornaviridae, Rhinovirus, Human rhinovirus A

Agent

Human rhinovirus 57

培養基

Strain

Ch47

Applications

Respiratory research

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format

frozen

Storage Conditions

?-70°C or colder

Comments

Virus growth is optimal at pH 6.8-7.3, in roller or rocker cultures incubated at approximately 33°C. All members of the rhinovirus group are ether resistant.

Effect on Host

Cytopathic effects (CPE) in culture include rounding and sloughing of cells

?CPE in culture include cell rounding and sloughing

Growth Medium

ATCC^® Medium 30-2003: Eagle's Minimum Essential Medium (EMEM)
Fetal Bovine Serum

Recommended Host

Production Host - H1-HeLa cells (ATCC CRL-1958)

Recommended Host: ?H1 HeLa (ATCC^? CRL-1958™)

Alternate Host(s) - WI-38 cells (ATCC CCL-75); MRC-5 cells (ATCC CCL-171); HeLa cells (ATCC CCL-2); other human cells

Growth Conditions

Host - H1 HeLa cells (ATCC CRL-1958)

Medium - EMEM (ATCC 30-2003) + 2% FBS (ATCC 30-2020)

Infection conditions - For best results, plate cells 24-48 hours in advance. Infect at about 80% confluence using a multiplicity of infection (MOI) of 0.1 to 1.0. Adsorb virus to cells in a small volume for one hour. After adsorbtion, feed with sufficient medium to maintain cells and incubate for 1 to 7 days at 33 °C, with rocking, in a humidified atmosphere with 5% CO2 . Harvest culture fluid or fluid and cells when entire culture displays cytopathic effects.

Preservation - Freeze as quickly as possible and store at -70°C or colder.

Name of Depositor

NIAID, ATCC

Special Collection

ATCC

Source

Derived by passage of NIAID reagent V-112-001-021 (ATCC VR-1167) at ATCC

References

NIAID Catalog of Research Reagents 1978-1980. S. Cunningham, ed. US Department of Health Education and Welfare, Public Health Service, National Institutes of Health; DHEW Pulication No. (NIH) 78-899, Bethesda, MD.

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