DESCRIPTION OF VECTOR COMPONENT: Name of vector: pHC79 Intact vector size: 6.430 Type of vector: cosmid Vector end: BamHI Vector end: BamHI Cloning sites: EcoRI ClaI BamHI SalI EcaI PstI Polylinker sites: Construction: pBR322, Charon 4A Host range: Escherichia coli Features (with orientation and position when available): marker(s): ampR, tetR replicon: pMB1 terminator: none Cross references: DNA Seq. Acc.: L08873
Insert
DNA: genomic
DESCRIPTION OF INSERT COMPONENT: Genome: Saccharomyces cerevisiae Gene symbol: SEC7 Gene name: protein transport protein Contains complete coding sequence?: Y Chromosome: IV Genome: Saccharomyces cerevisiae Gene symbol: SUP35 Gene name: omnipotent suppressor protein 2 Contains complete coding sequence?: Y Chromosome: IV Genome: Saccharomyces cerevisiae Gene symbol: ARGR3 Gene name: arginine metabolism regulation Contains complete coding sequence?: Y Chromosome: IV Genome: Saccharomyces cerevisiae Gene symbol: NGG1 Contains complete coding sequence?: Y Chromosome: IV Genome: Saccharomyces cerevisiae Gene symbol: UBC1 Gene name: ubiquitin-conjugating enzyme Contains complete coding sequence?: Y Chromosome: IV Genome: Saccharomyces cerevisiae Gene symbol: SDH4 Gene name: succinate dehydrogenase membrane anchor subunit Contains complete coding sequence?: Y Chromosome: IV Genome: Saccharomyces cerevisiae Gene symbol: CDC1 Contains complete coding sequence?: Y Chromosome: IV Genome: Saccharomyces cerevisiae Gene symbol: TCP20 Gene name: TriC chaperonin Contains complete coding sequence?: Y Chromosome: IV Genome: Saccharomyces cerevisiae Gene symbol: SLY1 Contains complete coding sequence?: Y Chromosome: IV Strain: AB972 (ATCC 76269) Type of DNA: genomic Insert end: MboI Insert end: MboI Insert size (kb): Cross references: DNA Seq. Acc.: Z46727
Gene product: protein transport protein [CDC1]
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Shipping Information
Distributed: frozen, uncharacterized
Comments
IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture.
