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pSLF101

規(guī)格:

價格:

編號:B222335

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規(guī)格:

凍干粉

斜面

甘油

平板

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產(chǎn)品名稱

pSLF101

商品貨號

B222335

Designations

pSLF101

Depositors

SL Forsburg

培養(yǎng)基

Biosafety Level

Host

Distribution host: Escherichia coli HB101 (ATCC 33694)

Vector Information

Size (kb): 6.8000001907348630

DESCRIPTION OF VECTOR:
Intact vector size: 6.800
Type of vector: phagemid
Cloning sites: SphI PstI SalI BamHI SmaI KpnI HpaI_
Polylinker sites: SphI PstI SalI BamHI SmaI KpnI HpaI
Other unique sites: ScaI SspI
Host range: Schizosaccharomyces pombe; Escherichia coli
Features (with orientation and position when available):
replicon: ars1
replicon: pMB1
marker(s): LEU2
operator: tet, <-
promoter: CaMV, <-
marker(s): ampR, <-

Vector: pSLF101 (phagemid)

Promoters: Promoter CaMV

Marker(s):LEU2,ampR

Construct size (kb): 6.800000190734863

Features: marker(s): LEU2
marker(s): ampR
operator: tet
promoter: CaMV
replicon: ars1
replicon: pMB1

Applications

expression vector

Comments

Restriction digests of the clone give the following sizes (kb): HindIII--4.6, 2.2; BamHI--6.8; EcoRI--3.9, 1.2.

The vector contains the constitutive CaMV promoter adjacent to the tet operator.

The vectors pSLF101 and pSLF102 differ in that pSLF101 has LEU2 marker and pSLF102, ura4+.

A tet repressor must be supplied to regulate expression. Schizosaccharomyces pombe FY191 (ATCC 201437) has a tet repressor gene and integrating vector pSLF104 (ATCC 87621) contains the sup3-5 marker and tet repressor under adh promoter.

Media

ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Growth Conditions

Temperature: 37.0°C

References

Forsburg SL. Comparison of Schizosaccharomyces pombe expression systems. Nucleic Acids Res. 21: 2955-2956, 1993. PubMed: 8332516