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pRabGGT-A

規格:

價格:

編號:B222522

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

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產品名稱

pRabGGT-A

商品貨號

B222522

Designations

pRabGGT-A

GenBank Number

L13722

培養基

Species

Rattus norvegicus, rat

Depositors

DA Andres

Vector

Construct size (kb): 7.5

Insert

DNA: cDNA

Insert lengths(kb): 4.5

Gene product: choroideremia(REP-1, Rab escort protein 1, tapeto-choroidal dystrophy, progressive choroidemia) [CHM]

Insert Size (kb)

4.5

Media

ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information

Distributed: frozen

Comments

Restriction digests of the clone give the following sizes (kb): XhoI/XbaI--4.5, 2.95, 0.3 +smaller; EcoRI--3.3, 3.1, 1.6; EcoRV--6.3, 1.7; NdeI--6.4, 1.55; HindIII--4.4, 3.5.

Restriction digests of the clone give the following sizes (kb): EcoRI--3.2, 2.9, 1.3; EcoRV--5.7, 1.67; NdeI--5.9, 1.475.

This is the homolog of the protein which, when defective in humans, results in choroideremia (CHM).

Transcription from the T7 promoter gives the sense strand.

References

Andres DA, et al. cDNA cloning of component A of Rab geranylgeranyl transferase and demonstration of its role as a Rab escort protein. Cell 73: 1091-1099, 1993. PubMed: 8513495

Douglas A Andres, personal communication

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