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8AC

規格:

價格:

編號:B222608

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

8AC

商品貨號

B222608

Designations

8AC

GenBank Number

M60493

培養基

Species

Mus musculus, mouse

Depositors

T Yorifuji, AL Beaudet

Vector

Construct size (kb): 6.820000171661377

Insert

DNA: cDNA

Insert lengths(kb): 3.880000114440918

Gene product: cystic fibrosis transmembrane conductance regulator [Cftr]

Insert Size (kb)

3.880

Media

ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information

Distributed: freeze-dried

Comments

Restriction digests of the clone give the following sizes (kb): BamHI--4.6, 1.5, 0.65; EcoRI--5.4, 1.45; HindIII--3.2, 2.3, 0.85, 0.7; PstI--6.9; PstI/XhoI--3.9, 2.9.

The insert contains the following restriction sites (nt from the 5' end): HindIII--85, 856, 1539, 3792; SalI--568; BamHI--1600, 2252; EcoRI--1510.

The insert extends from nt 2438-6304 of the reported sequence, including exons 13 through the 3' end. Also includes a short (14 nt) poly(A) tail not in the sequence record.

Transcription from the T3 promoter gives the sense strand.

Classification

Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod

References

Oliva D, et al. Complete structure of the human gene encoding neuron-specific enolase. Genomics 10: 157-165, 1991. PubMed: 2045099

Tohru Yorifuji, personal communication

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