黄色小说视频-日本少妇高潮抽搐-国内自拍av-天堂中文资源在线-蜜桃视频在线入口www-91久久综合-亚洲视频在线一区-日日夜夜拍-国产精品资源在线观看-欧美激情国产精品免费-人妻少妇久久中文字幕-成人免费无码大片a毛片-香蕉污视频在线观看-日本电影成人-国内精品久久99人妻无码

歷史搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購物車 1 種商品 - 共0元
當前位置: 首頁 > ATCC代理 > VMM425 B222637
VMM425
VMM425
規格:
價格:
編號:B222637
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
VMM425
商品貨號
B222637
Organism
Homo sapiens, human
Tissue
Melanoma, Pelvic Soft Tissue Metastasis
培養基
Cell Type
Melanocyte
Product Format
frozen
Morphology
Epithelial-like
Culture Properties
adherent
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
Melanoma, Stage IV; malignant
Age
60
Gender
Male
Ethnicity
Caucasian
Applications
Drug screening
Development of targeted therapy
Development of combination therapy
Tumor vaccine development
Storage Conditions
liquid nitrogen vapor phase
Images
ATCC CRL-3231 Cell Micrograph
Derivation
Derived from a pelvic soft tissue recurrence. Established from digest of Right Pelvic Tumor Metastasis in PETI Medium.
Clinical Data
Primary Site: Left Foot (Plantar); Metastatic Site: Pelvic Soft Tissue
HLA Typing
A2,A31,B7,B51,Cw7?,Cw16?,DR4(DRB1*0404),[DR11(DRB1*1101) or DR13(DRB1*131401)],DR52(DRB3*0202),DR53(DRB4*0103)
Comments
NRAS Mutation: Q61L Q61R
CDKN2A: wt
BRAF: wt

PET Scan +
Complete Growth Medium
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
Subculturing

Volumes are for a T-75 flask; Adjust accordingly

  1. Remove and discard the cell culture medium from the flask.
  2. Rinse the cell monolayer with Dulbecco’s PBS without calcium or magnesium and remove.
  3. Add 3 to 4 ml of the trypsin-EDTA solution, rotate flask to rinse cell monolayer, remove trypsin solution, and incubate at 37oC.
  4. Once the cells appear to be detached, add 10 ml of complete growth medium with a pipette to the cell suspension to inactivate the trypsin. Gently wash any remaining cells from the growth surface of the   flask. Check the  cells with the microscope to be sure that most (>95%) are single cells. If cell clusters are apparent, continue to disperse the cells with gentle pipetting.
  5. Subculture as necessary.
  6. Place the flask back into the incubator. Examine the culture the following day to ensure the cells have reattached and are actively growing.
  7. Repeat when cells reach ~ 50-75% confluence.
CELLS DO NOT GET CONFLUENT
Cryopreservation
Fetal bovine serum, 90%; DMSO, 10%
Culture Conditions
Temperature: 37°C
Atmosphere: air, 95%; carbon dioxide (CO2 ), 5%
STR Profile
Amelogenin: X     
D5S818: 11
D13S317: 9,13 
D7S820: 9
D16S539: 12
vWA: 17,19
TH01: 8,9.3  
TPOX: 8,12
CSF1PO: 12

Sterility Tests

Pass

Population Doubling Level (PDL)
unknown
Population Doubling Capacity
unknown
Name of Depositor
Craig L. Slingluff, Jr. M.D.
Passage History

Original deposit at one passage from the primary culture

Year of Origin
2007
References

Molhoek K, et al. Comprehensive analysis of RTK activation in human melanomas reveals autocrine signaling through IGF-1R. Melanoma Res 21(4): 274–284, 2011. PubMed: 21654344

  • 王經理
    手機號：17280875617
    QQ：1438578920
  • 胡經理
    手機號：13345964880
    QQ：2438244627
  • 周經理
    手機號：17757487661
    QQ：1296385441
  • 于經理
    手機號：18067160830
    QQ：2088210172
  • 沈經理
    手機號：19548299266
    QQ：2662369050
  • 李經理
    手機號：13626845108
    QQ：972239479