黄色小说视频-日本少妇高潮抽搐-国内自拍av-天堂中文资源在线-蜜桃视频在线入口www-91久久综合-亚洲视频在线一区-日日夜夜拍-国产精品资源在线观看-欧美激情国产精品免费-人妻少妇久久中文字幕-成人免费无码大片a毛片-香蕉污视频在线观看-日本电影成人-国内精品久久99人妻无码

歷史搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購物車 1 種商品 - 共0元
當前位置: 首頁 > ATCC代理 > pUK4134 B222674
pUK4134
pUK4134
規格:
價格:
編號:B222674
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
pUK4134
商品貨號
B222674
Designations
pUK4134
Depositors
SC Straley
培養基
Biosafety Level
1
Vector Information
Size (kb): 7.0000000000000000
Vector: pUK4134 (plasmid)
Construction: pRTP1, pJM703.1
Marker(s):ampR,strS
Construct size (kb): 6.699999809265137
Features: marker(s): ampR
marker(s): strS
other: lambda cos site
replicon: R6K
replicon: oriT
Applications
contains sequence origin of transfer replication
contains sequence ribosomal protein S12
integrating vector
shuttle vector
vector permitting positive selection for integration
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--6.7, 0.5; EcoRI--6.4, 0.8; EcoRV--7.2; SalI--7.2.
RP6 is a suicide replicon in hosts not producing the pir protein.
RpsL (streptomycin sensitive) is dominant to streptomycin resistance, so loss of the plasmid can identified by streptomycin resistance and ampicillin sensitivity.
Suicide, gene replacement vector for Yersiniae and other gram-negative hosts that permits positive selection for loss of the plasmid after allelic exchange.
The vector also contains cos sites and oriT, and can be used for cosmid cloning or conjugative transfer.
The order of the major features in the plasmid is: EcoRV - R6K ori - oriT - SalI - cos - rpsL - ampR.
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Skrzypek E, et al. New suicide vector for gene replacement in Yersiniae and other gram-negative bacteria. Plasmid 29: 160-163, 1993. PubMed: 8469722

Stibitz S, et al. The construction of a cloning vector designed for gene replacement in Bordetella pertussis. Gene 50: 133-140, 1986. PubMed: 2884169

Miller VL, Mekalanos JJ. A novel suicide vector and its use in construction of insertion mutations: osmoregulation of outer membrane proteins and virulence determinants in Vibrio cholerae requires toxR. J. Bacteriol. 170: 2575-2583, 1988. PubMed: 2836362

Shipped
freeze-dried
  • 王經理
    手機號：17280875617
    QQ：1438578920
  • 胡經理
    手機號：13345964880
    QQ：2438244627
  • 周經理
    手機號：17757487661
    QQ：1296385441
  • 于經理
    手機號：18067160830
    QQ：2088210172
  • 沈經理
    手機號：19548299266
    QQ：2662369050
  • 李經理
    手機號：13626845108
    QQ：972239479