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pSY2501

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價格:

編號:B222706

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

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凍干粉

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甘油

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產品名稱

pSY2501

商品貨號

B222706

Designations

pSY2501

GenBank Number

I02222

培養基

Species

Homo sapiens, human

Depositors

Cetus Corp., A Belt, Cetus Corp.

Applications

produces protein interferon, beta 1, fibroblast

Vector

Construct size (kb): 5.0

DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pTRP3
Intact vector size: 4.460
Type of vector: plasmid
Vector end: HindIII
Vector end: BamHI
Cloning sites: HindIII
Polylinker sites:
Other unique sites: BamHI
Construction: pVH153, pBR322
Host range: Escherichia coli
Features (with orientation and position when available):
promoter: trp, ->
ribosome-binding site: , ->
restriction site: HindIII, ->
marker(s): tetR, ->
replicon: pMB1
marker(s): ampR, <-
Cross references:

Insert

DNA: cDNA

DESCRIPTION OF INSERT COMPONENT:
Genome: human
Gene symbol: IFNB1
Other gene symbols: IFNB
Genomic copy number: unique
Gene name: interferon, beta 1, fibroblast
Contains complete coding sequence?: U
Chromosome: 9; Localization: 9 p22
Type of DNA: cDNA
Insert end: HindIII
Insert end: XhoII
Insert size (kb): 0.5
Cross references: DNA Seq. Acc.: I02222

Insert lengths(kb): 0.5

Gene product: interferon, beta 1, fibroblast [IFNB]

Target Gene: interferon, beta 1, fibroblast

Insert Size (kb)

0.5

Media

ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information

Distributed: freeze-dried

Comments

Contains an Escherichia coli trp promoter regulating expression of complete coding sequence. Mutated codon changes Cys17 to Ser17. Contains 4 internal HinfI and 1 PstI sites.

Constructed by ligating HindIII/XhoII fragment into HindIII/BamHI-cut vector (destroying XhoII/BamHI site); HindIII/XhoII digest gives nearly complete insert.

References

Lin LS, et al. Human recombinant interleukin-2-muteins. US Patent 4,518,584 dated May 21 1985

Gelfand DH, et al. Polypeptide expression using a portable temperature sensitive control cassette with a positive retroregulatory element. US Patent 4,666,848 dated May 19 1987

Disclosure

This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.

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