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pGC3 [BB915]

規(guī)格:

價格:

編號:B222730

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規(guī)格:

凍干粉

斜面

甘油

平板

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產品名稱

pGC3 [BB915]

商品貨號

B222730

Designations

pGC3 [BB915]

Depositors

JD Boeke

培養(yǎng)基

Biosafety Level

Vector Information

Size (kb): 7.4609999656677250

Vector: pGC3 (phagemid)

Promoters: Promoter for in vitro transcription T7

Construction: pRS413

Marker(s):ampR,HIS3,MET15

Construct size (kb): 7.460999965667725

Features: insert detection: lacZ'
marker(s): HIS3
marker(s): MET15
marker(s): ampR
promoter: lac
promoter for in vitro transcription: T3
promoter for in vitro transcription: T7
replicon: ARSH4
replicon: f1
replicon: pMB1
MCS: BamHI...KpnI
MCS: SacI...EagI
centromere: CEN6

Applications

YC-type (centromeric) shuttle vector

vector containing primer sites useful for sequencing

vector permitting RNA synthesis in vitro

vector permitting production of single-stranded DNA

vector permitting visual detection of recombinants O-acetylhomoserine sulfhydrylase

Comments

Restriction digests of the clone give the following sizes (kb): BamHI--7.2; EcoRI--4.8, 1.4, 0.94; PvuII--4.4, 2.7.

The insert contains the following restriction sites (approximate kb from the 5' end): XbaI--0.72; EcoRI--0.99, 2.37; HindIII--1.70; ScaI--1.96.

An SpeI digest removes an intact 1.334 kb MET15 cassette, that includes flanking sequences of 577 bp at the 5' end and 580 bp at the 3' end).

MET15, MET17 and MET25 are synonymous.

The sequence of the PCR-generated MET15 cassette has not been determined to eliminate the possibility of PCR error. It was selected based on the described functional assay.

Media

ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Growth Conditions

Temperature: 37.0°C

References

Cost GJ, Boeke JD. A useful colony colour phenotype associated with the yeast selectable/counter-selectable marker MET15. Yeast 12: 939-941, 1996. PubMed: 8873447

Sikorski RS, Hieter P. A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae. Genetics 122: 19-27, 1989. PubMed: 2659436

Shipped

freeze-dried

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