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HF [hSNF2L]
HF [hSNF2L]
規格:
價格:
編號:B222926
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
HF [hSNF2L]
商品貨號
B222926
Designations
HF [hSNF2L]
GenBank Number

M88163

培養基
Species
Homo sapiens, human
Vector
Construct size (kb): 6.800000190734863
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pGEM-3Zf(-)
Intact vector size: 3.200
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: EcoRI SacI KpnI AvaI SmaI BamHI XbaI SalI AccI HincII PstI
SphI HindIII
Polylinker sites: T7 - EcoRI SacI KpnI AvaI SmaI BamHI XbaI SalI AccI HincII
PstI SphI HindIII - SP6
Host range: Escherichia coli
Features (with orientation and position when available):
insert detection: lacZ'
marker(s): ampR
promoter: lac, SP6, T7
replicon: pMB1, f1
Cross references:
Insert
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: SNF2L
Genomic copy number: unique
Gene name: SNF2 (sucrose nonfermenting, yeast, homolog)-like
Contains complete coding sequence?: Y
Chromosome: X; Localization: X q25
Tissue: fibroblast
Type of DNA: cDNA
Insert end: Modification: EcoRI linkers
Insert end: Modification: EcoRI linkers
Insert size (kb): 3.6
Cross references: DNA Seq. Acc.: M88163
Insert lengths(kb): 3.599999904632568
Tissue: fibroblast
Gene product: SNF2 (sucrose nonfermenting, yeast, homolog)-like [SNF2L]
Insert Size (kb)
3.600
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--3.2, 2.6, 0.7, 0.3; HindIII--4.0, 1.4, 1.3, 0.2; PvuII--2.7 (doublet), 1.35; SacI--5.5, 0.9, 0.45; SalI--4.0, 2.2, 0.6.
The insert contains the following restriction sites (bp from the 5' end): EcoRI--2681, 2938; HindIII--871, 2122, 3478; NcoI--2933; NdeI--434; PstI--998, 2006; PvuII--2603; SacI--2299, 3180; SalI--900, 1496; XhoI--2558.
Contains the complete coding sequence (nt 142-3072).
Sequence shows strong homology to the Saccharomyces cerevisiae transcription activator genes SNF2/SWI2 and STH1.
The insert detects a 4.4 kb transcript in the following human tissues: heart, brain, placenta, lung, liver, skeletal muscle and kidney.
References

Okabe I, et al. Cloning of human and bovine homologs of SNF2/SWI2: a global activator of transcription of yeast S. cerevisiae. Nucleic Acids Res. 20: 4649-4655, 1992. PubMed: 1408766

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