X03363
Name of vector: pcD
Intact vector size: 3.000
Type of vector: plasmid
Cloning sites: XhoI BamHI
Polylinker sites:
Construction: pBR322, SV40
Host range: vertebrate cells; Escherichia coli
Features (with orientation and position when available):
Enhancer: SV40
Marker(s): ampR
Promoter: SV40 early, late
Replicon: pMB1, SV40
Terminator: SV40 late
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
The insert is not readily excised from the vector (flanked by GC and AT tails). StuI cuts twice in the insert but not in the vector. The vector is cut with HindIII. A StuI/HindIII double digest gives a 3.4 kb fragment specific to c-erbB-2.
Zhang X, et al. Amplification and rearrangement of c-erb B proto-oncogenes in cancer of human female genital tract. Oncogene 4: 985-989, 1989. PubMed: 2569708
Yamamoto T, et al. Similarity of protein encoded by the human c-erb-B-2 gene to epidermal growth factor receptor. Nature 319: 230-234, 1986. PubMed: 3003577
Fukushige S, et al. Localization of a novel v-erbB-related gene, c-erbB-2, on human chromosome 17 and its amplification in a gastric cancer cell line. Mol. Cell. Biol. 6: 955-958, 1986. PubMed: 2430175
Ishii S, et al. Characterization of the promoter region of the human c-erbB-2 protooncogene. Proc. Natl. Acad. Sci. USA 84: 4374-4378, 1987. PubMed: 2885835
