Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
This increased metastatic cell line was derived using an in vivo selection process of highly metastatic cells from a population of poorly metastatic tumor cells, A375 (ATCC® No. CRL-1619™) and A375P a donation from from Dr. Isaiah Fidler (Texas MD Anderson Cancer Center, for Set F cells).
A375P and CRL-1619 were used to create more lung metastases cell lines when injected into mice.The A375-M1 (ATCC® No. CRL-3222™) cell line was derived by i.v. injection of A375 cells into nude mice. Lung metastases were harvested and amplified in vitro as cell lines. The A375-M1 cell line was reinjected into mice for a second round of selection, lung metastases harvested and amplified in vitro as A375-M2 (ATCC® No. CRL-3223™) cells. The A375-M1 and A375-M2 cell lines were transfected with a plasmid containing the ecotropic receptor for murine retrovirus and selected for neomycin resistance. This is useful for RNAse protection assays.
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Clark EA, et al. Genomic analysis of metastasis reveals an essential role for rhoC. Nature 406:532-535, 2000. PubMed: 1095231
