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pHE1
pHE1
規(guī)格:
價(jià)格:
編號(hào):B223064
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
pHE1
商品貨號(hào)
B223064
Designations
pHE1
Species
Homo sapiens, human
培養(yǎng)基
Depositors
GE Mark
Vector
Construct size (kb): 6.099999904632568
Insert
DNA: cDNA
Insert lengths(kb): 1.799999952316284
Tissue: fetal liver
Gene product: murine leukemia viral (v-raf-1) oncogene homolog 1( Craf1 transforming gene, murine sarcoma 3611 oncogene 1, raf proto-oncogene serine/threonine protein kinase, v-raf-1 murine leukemia viral oncogene homolog 1, Oncogene RAF1) [RAF1]
Alleles: B1, A1, A2, B2
Insert Size (kb)
1.800
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments
EcoRI linkers added to cDNA before insertion; insert oriented with HindIII site of insert closer to PstI site of pBR322.
Restriction digests of the clone give the following sizes (kb): EcoRI--4.35, 1.8; BglII--6.2; HindIII--6.2.
Insert contains HindIII, NdeI, BglII, EcoRII, TaqI, HincII, XbaI, ApaI, PvuII, and AccI sites.
Recorded as not polymorphic by Collaborative Research, meaning either not polymorphic in 0.8% agarose gels, or difficult to work with.
References

Gribbin TE, et al. Association of a mature B cell leukemia with a 4p+ chromosomal abnormality: derivation and characterization of a cell line. Leukemia 3: 643-647, 1989. PubMed: 2548046

Bonner TI, et al. Structure and biological activity of human homologs of the raf/mil oncogene. Mol. Cell. Biol. 5: 1400-1407, 1985. PubMed: 2993863

Barbara Weiffenbach, personal communication

George E Mark, personal communication

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