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當前位置: 首頁 > ATCC代理 > pLamB2(21/1) B223286
pLamB2(21/1)
pLamB2(21/1)
規格:
價格:
編號:B223286
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
pLamB2(21/1)
商品貨號
B223286
Designations
pLamB2(21/1)
GenBank Number

J02930

培養基
Species
Mus musculus, mouse
Depositors
I Oberbaumer
Applications
The 1.9 kb StuI or 1.7 kb HindIII/SspI fragments are recommended for hybridization.
Vector
Construct size (kb): 5.199999809265137
Insert
DNA: cDNA
Insert lengths(kb): 2.517999887466431
Tissue: teratocarcinoma PYS-2/12 cell line
Gene product: laminin, gamma 1 (formerly LAMB2)(laminin, B2 polypeptide) [Lamb2]
Alleles: a, b, d, d
Insert Size (kb)
2.518
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): BamHI/EcoRI--2.6 (doublet); EcoRI--5.2; HindIII--3.4, 1.8; XbaI--3.6, 1.6; PstI--4.2, 1.0.
The insert contains the following restriction sites (approximate kb from the 5' end): StuI--0.02, 1.91; HindIII--0.78; XbaI--1.02; PstI--1.68; SacI--1.99; SspI--2.5.
Extends from nt 5036 to 7544 of the reported sequence, containing most of the 3' untranslated region.
The 1.9 kb StuI or 1.7 kb HindIII/SspI fragments are recommended for hybridization.
Classification
Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod
References

Oberbaumer I. New pUC-derived expression vectors for rapid construction of cDNA libraries. Gene 49: 81-91, 1986. PubMed: 2436975

Oberbaumer I. Blue libraries are beautiful. Focus 14: 72, 1992.

Durkin ME, et al. The primary structure of the mouse laminin B2 chain and comparison with laminin B1. Biochemistry 27: 5198-5204, 1988. PubMed: 3167041

Ilse Oberbaumer, personal communication

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