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pACYC177
pACYC177
規格:
價格:
編號:B223367
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
pACYC177
商品貨號
B223367
Designations
pACYC177
Depositors
SN Cohen
培養基
Other IDs

Nucleotide (GenBank) : VB0109 Cloning vector pACYC177.

Biosafety Level
1
Vector Information
Size (kb): 3.9400000572204590
Vector: pACYC177 (plasmid)
Construction: p15A, Tn903, Tn3
Marker(s):ampR,kanR
Construct size (kb): 3.940000057220459
Features: marker(s): ampR, kanR
replicon: p15A
Comments
Low copy number plasmid vector containing the replication system of miniplasmid p15A.
When HincII is nucleotide 1, Tn3 region extends from 3026-756 and beta-lactamase from 369-615. The promoter and part of the coding region for the Tn3 repressor (3025-3515) have been deleted. The Tn903 (1595-3025) region includes kanR (1922-2734).
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Chang AC, Cohen SN. Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid. J. Bacteriol. 134: 1141-1156, 1978. PubMed: 149110

Rose RE. The nucleotide sequence of pACYC177. Nucleic Acids Res. 16: 356, 1988. PubMed: 3340534

Shipped
frozen
Shipping Information

Shipped: ?Frozen glycerol stock of ?E. col containing the plasmid.

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