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當前位置: 首頁 > ATCC代理 > pUC-Cathepsin G [pUC-Cathepsin] B223437
pUC-Cathepsin G [pUC-Cathepsin]
pUC-Cathepsin G [pUC-Cathepsin]
規格:
價格:
編號:B223437
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
pUC-Cathepsin G [pUC-Cathepsin]
商品貨號
B223437
Designations
pUC-Cathepsin G [pUC-Cathepsin]
GenBank Number

J04990

培養基
Species
Homo sapiens, human
Depositors
TJ Ley
Vector
Construct size (kb): 5.699999809265137
Insert
DNA: genomic
Insert lengths(kb): 3.0
Tissue: lung fibroblast
Gene product: cathepsin G [CTSG]
Insert Size (kb)
3.0
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
This insert includes an internal HindIII site which maps to exon 5.
Restriction digests of the clone give the following sizes (kb): BamHI--5.7; HindIII--3.0, 2.7; EcoRI--3.0, 2.7; HincII--5.7.
A unique probe for cathepsin G can be made by purifying the 0.35 kb HindIII/EcoRI fragment derived from exon 5 and the 3' flanking region of this gene.
The insert is an EcoRI fragment that begins at position -76 with respect to the transcription initiation site and ends about 300 bp downstream of the poly(A) site.
The cathepsin G gene is divided into 5 exons and 4 introns, similar to several other serine proteases, including human neutrophilelastase, rat mast cell protease II, murine adipsin and murine and human cytotoxic T-cell serine proteases.
At high stringency, this probe hybridizes to a genomic BamHI fragment of 6.0 kb, a genomic BglII fragment of 8.5 kb, and a genomic EcoRI fragment of about 3.0 kb. There is also a polymorphic 4.3 kb BglII fragment.
References

Hohn PA, et al. Genomic organization and chromosomal localization of the human cathepsin G gene. J. Biol. Chem. 264: 13412-13419, 1989. PubMed: 2569462

Hanson RD, et al. Developmental regulation of the human cathepsin G gene in myelomonocytic cells. J. Biol. Chem. 265: 1524-1530, 1990. PubMed: 2295643

Timothy J Ley, personal communication

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