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pKSM711

規格:

價格:

編號:B223638

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

pKSM711

商品貨號

B223638

Designations

pKSM711

Depositors

K Ippen-Ihler, S Maneewannakul

培養基

Biosafety Level

Vector Information

Size (kb): 3.7409999370574950

Vector: pKSM711 (phagemid)

Promoters: Promoter for in vitro transcription T7lac

Construction: pKSM710, pUC4-KIXX

Marker(s):kanR

Construct size (kb): 3.740999937057495

Features: insert detection: lacZalpha
marker(s): kanR
promoter for expression: lacUV5
promoter for in vitro transcription: T7lac
replicon: fd
replicon: pMB1
MCS: BamHI...EcoRI
transcription terminator: T7
transcription terminator: fd
transcription terminator: rrnB

Applications

expression vector

vector permitting RNA synthesis in vitro

vector permitting production of single-stranded DNA

vector permitting visual detection of recombinants

Comments

Restriction digests of the clone give the following sizes (kb): BamHI--3.7; EcoRI--3.7; PstI--3.7.

Vector contains the following restriction sites (approximate kb from nt 1): BamHI--0.34; BglII--3.42; EcoRI--0.39; NcoI--0.33, 2.82; PstI--3.20; PvuI--0.51; PvuII--0.48, 3.15, 3.51.

Differs from pKSM712 (ATCC 87158) only in the orientation of the kanamycin resistance gene.

Expression vector.

Media

ATCC^® Medium 1236: LB Medium (ATCC medium 1065) with 25 mcg/ml kanamycin

Growth Conditions

Temperature: 37.0°C

References

Maneewannakul S, et al. The pKSM710 vector cassette provides tightly regulated lac and T7lac promoters and strategies for manipulating N-terminal protein sequences. Plasmid 31: 300-307, 1994. PubMed: 8058823

Shipped

freeze-dried

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