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pCyR-1 [PL-1021]
pCyR-1 [PL-1021]
規(guī)格:
價格:詢價
編號:B223733
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
pCyR-1 [PL-1021]
商品貨號
B223733
Designations
pCyR-1 [PL-1021]
Species
Cymbidium ringspot virus
培養(yǎng)基
Depositors
DSM
Vector
Construct size (kb): 6.5
Insert
DNA: cDNA
Insert lengths(kb): 3.799999952316284
Gene product: coat protein; 22K protein
Insert Size (kb)
3.800
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--3.9, 2.9; BamHI/HindIII--3.2, 2.7, 0.6; PstI--3.8, 2.7; HindIII--6.5; BamHI--3.5, 3.0.
The insert includes the following sites (approximate kb from the 5' end of the insert): BamHI--0.27; EcoRI--1.1; ApaI--1.6; BglII--2.2; KpnI--2.4.
Encodes about 80% of the virus genome from the 3' end.
Hybridizes to three RNAs from infected plant tissue: a genomic RNA (about 4.7 kb), a 2.1 kb RNA and a 1.0 kb RNA.
Classification
Tombusvirus
References

Russo M, et al. Complementary DNA cloning and characterization of cymbidium ringspot virus RNA. J. Gen. Virol. 69: 401-406, 1988.

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