Tissue: kidney, Madin-Darby Bovine Kidney (MDBK) cell line
Gene product: annexin I (lipocortin I) [ANX1]
Insert Size (kb)
1.338
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Shipping Information
Distributed: freeze-dried
Comments
The insert contains the following restriction sites, in nt from the 5' end according to the EMBL record: EcoRI--19, BamHI--63; PstI--260; SacI--371; BglII--489.
Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 1.3, 0.02; BamHI--4.2, 0.1; SphI--4.3; PstI--4.0, 0.3; HindIII--4.3.
Use of T3 RNA polymerase will yield the sense strand; T7 RNA polymerase will yield the complementary/antisense strand.
