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pHBI59
pHBI59
規格:
價格:
編號:B223877
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
pHBI59
商品貨號
B223877
Designations
pHBI59
Species
Homo sapiens, human
培養基
Applications
Mapped to the pericentromeric region by multipoint linkage analysis.
This probe has been used to detect loss of heterozygosity at MEN1. Some tumors retain this sequence.
Also detects a TaqI polymorphism not yet verified by pedigree analysis.
Vector
Construct size (kb): 4.289999961853027
Insert
DNA: genomic
Insert lengths(kb): 1.600000023841858
Gene product: DNA Segment, single copy [D11S146]
Alleles: A1, B1, B2, A2
Insert Size (kb)
1.600
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--2.8, 0.97, 0.52.
An EcoRI/HindIII double digest shows insert fragment sizes of 0.9 and 0.4 kb.
Mapped to the pericentromeric region by multipoint linkage analysis.
This probe has been used to detect loss of heterozygosity at MEN1. Some tumors retain this sequence. MEN1 maps between PYGM and D11S146.
Detects human genomic restriction fragments of the following sizes (kb): BssHI--600; ClaI--850, 700, 600, 450; NruI--1500; SacII--600; SalI--950, 800, 600.
Codominant segregation of the MspI polymorphism has been demonstrated in 50 3-generation families. Also detects a TaqI polymorphism not yet verified by pedigree analysis.
Enzyme(s) not detecting polymorphism: BglII, PstI, PvuII, RsaI.
References

Nakamura Y, et al. Isolation and mapping of a polymorphic DNA sequence (pHBI59) on chromosome 11 [D11S146]. Nucleic Acids Res. 16: 10404, 1988. PubMed: 2461547

Nakamura Y, et al. Localization of the genetic defect in multiple endocrine neoplasia type I within a small region of chromosome 11. Am. J. Hum. Genet. 44: 751-755, 1989. PubMed: 2565085

Szepetowski P, et al. D11S146 and BCL1 are physically linked but can be discriminated by their amplification status in human breast cancer. Genomics 10: 410-416, 1991. PubMed: 2071147

Nakamura Y, et al. Isolation and mapping of a polymorphic DNA sequence pHBI59 on chromosome 11 [D11S146]. Nucleic Acids Res. 16: 376, 1988. PubMed: 2448753

Yusuke Nakamura, personal communication

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