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pSC161RM O/P17
pSC161RM O/P17
規格:
價格:
編號:B223879
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
pSC161RM O/P17
商品貨號
B223879
Designations
pSC161RM O/P17
Species
Acinetobacter calcoaceticus (Beijerinck) Baumann et al.
培養基
Depositors
New England Biolabs, Inc., I Schildkraut, New England Biolabs, Inc.
Applications
in suitable host, produces protein modification methylase AccI
in suitable host, produces protein restriction endonuclease AccI
Insert
DNA: genomic
Insert lengths(kb): 4.199999809265137
Gene product: restriction endonuclease AccI [hsdM]
Target Gene: modification methylase AccI, restriction endonuclease AccI
Insert Size (kb)
4.200
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments
Restriction digests of the clone give the following sizes (kb): XbaI/PstI--4.5, 4.0, 2.6.
The ClaI fragment (which includes pBR322 ClaI/EcoRI sequence) contains the following restriction sites (approximate kb from the vector-derived end): EcoRI--0.02, 1.8; XmnI--0.9, 3.9; HindIII--3.3, 3.5; BanII--4.1; KpnI--4.1.
To optimize production, the plasmid should be transformed into E. coli MM294, grown at 30C to late log phase, and then shifted to 42C for an additional 3 hr.
The vector is pUC19 modified by insertion of a 4.3 kb BamHI fragment containing bacteriophage lambda N, PR, cI857, and PR sequences. The PL promoter drives transcription of the insert.
Distributed in aliquots of 100 ng dried.
References

Chen S, Wilson GG. Method for producing the AccI restriction endonuclease and methylase. US Patent 5,004,691 dated Apr 2 1991

Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
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