Vector Information
Size (kb): 7.3000001907348630
DESCRIPTION OF VECTOR:
Intact vector size: 7.300
Type of vector: plasmid
Cloning sites: EcoRI
Polylinker sites:
Construction: pRS424
Host range: Saccharomyces cerevisiae; Escherichia coli
Features (with orientation and position when available):
marker(s): TRP1, ->, 468-1142
replicon: f1, ->, 1241-1707
insert detection: lacZ', <-
promoter for in vitro transcription: T7, ->, 1867-1886
restriction site: SalI
terminator: RPR1, <-
restriction site: EcoRI
promoter: SUP4 tRNA intragenic promoter, <-
restriction site: BamHI
promoter for in vitro transcription: T3, <-
promoter: lac, <-
replicon: pMB1
marker(s): ampR, <-
replicon: 2 micron
Vector: pIIIEx424 tRNA (plasmid)
Promoters: Promoter for in vitro transcription T7
Construction: pRS424
Marker(s):TRP1,ampR
Construct size (kb): 7.300000190734863
Features: insert detection: lacZ'
marker(s): TRP1
marker(s): ampR
promoter: SUP4 tRNA intragenic promoter
promoter: lac
promoter for in vitro transcription: T3
promoter for in vitro transcription: T7
replicon: 2 micron
replicon: f1
replicon: pMB1
restriction site: BamHI
restriction site: EcoRI
restriction site: SalI
terminator: RPR1
