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pMuTNF [0169]

規格:

價格:

編號:B223907

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

pMuTNF [0169]

商品貨號

B223907

Designations

pMuTNF [0169]

GenBank Number

M11731

培養基

Species

Mus musculus, mouse

Depositors

B Fennie

Vector

Construct size (kb): 5.5

Insert

DNA: cDNA

Insert lengths(kb): 1.10099995136261

Tissue: monocyte-macrophage PU5-1.8 cell line (TIB 61)

Gene product: tumor necrosis factor, alpha (cachectin) [Tnfa]

Insert Size (kb)

1.101

Media

ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information

Distributed: freeze-dried

Comments

Restriction digests of the clone give the following sizes (kb): BamHI--5.6; BglI--2.4, 1.6, 1.05, 0.45; EcoRI--4.5, 1.15; PvuII-- 2.8, 2.5, 0.3; SacI--5.6.

The insert contains the following restriction sites (nt from the 5' end): BglI--79, 389; SacI--709; PvuII--752.

The insert is the 5' EcoRI fragment from clone lambda6, and contains the 5' untranslated region, the complete coding sequence, and the 3' untranslated region up to the naturally occurring EcoRI site at nt 1105.

Classification

Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod

References

Pennica D, et al. Cloning and expression in Escherichia coli of the cDNA for murine tumor necrosis factor. Proc. Natl. Acad. Sci. USA 82: 6060-6064, 1985. PubMed: 3898078

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