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pRBP4
pRBP4
規格:
價格:
編號:B224030
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
pRBP4
商品貨號
B224030
Designations
pRBP4
GenBank Number

X00129

培養基
Species
Homo sapiens, human
Depositors
GP Albaugh
Vector
Construct size (kb): 4.639999866485596
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pCRII
Intact vector size: 3.900
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: TA cloning between EcoRI sites
Polylinker sites: NsiI HindIII KpnI SacI BamHI SpeI BstXI EcoRI EcoRI EcoRV
BstXI NotI AvaI XmaIII XhoI NsiI XbaI ApaI
Host range: Escherichia coli
Features (with orientation and position when available):
insert detection: lacZ'
marker(s): ampR, kanR
promoter: lac, SP6, T7
replicon: pMB1
Cross references:
Insert
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: RBP4
Genomic copy number: unique
Gene name: retinol-binding protein 4, interstitial
Contains complete coding sequence?: Y
Chromosome: 10; Localization: 10 q23-q24
Tissue: liver
Type of DNA: cDNA
Insert end: Modification: EcoRI linkers
Insert end: Modification: EcoRI linkers
Insert size (kb): 0.736
Cross references: DNA Seq. Acc.: X00129
Insert lengths(kb): 0.7360000014305115
Tissue: liver
Gene product: retinol-binding protein 4, interstitial [RBP4]
Insert Size (kb)
0.736
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--3.9, 0.74; PstI--3.3, 1.2, 0.29; SalI--4.8; NcoI--3.0, 1.75; AvaI--4.1, 0.40, 0.30.
Constructed by amplification using primers annealing between nt 46 and 756 of the published sequence. Alignment of this sequence with the previous requires 3 gaps and 5 substitutions, and the reading frame is not maintained.
The insert contains the following restriction sites (bp from the 5' end): AvaI--163, 481; NcoI--146; NotI--61; PstI--178; SalI--387; SmaI--481.
Contains the complete coding sequence (nt 52-648 of GenBank accession X00129).
Transcription from the T7 promoter gives the sense strand.
References

Colantuoni V, et al. Cloning and sequencing of a full length cDNA coding for human retinol-binding protein. Nucleic Acids Res. 11: 7769-7776, 1983. PubMed: 6316270

Albaugh GP, Smith JC. Amplification, cloning and partial characterization of human liver retinol-binding protein cDNA. FASEB J. 7: A733, 1993.

George P Albaugh, personal communication

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