contains easily purifiable cassette(s) for construction
Comments
This plasmid is unstable when transformed into C600 galK-.
Contains an easily purifiable trp-lac hybrid promoter which can be used to construct expression vectors.
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References
Russell DR, Bennett GN. Construction and analysis of in vivo activity of E. coli promoter hybrids and promoter mutants that alter the -35 to -10 spacing. Gene 20: 231-243, 1982. PubMed: 6299890
