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HRRM1 [STSG-10564]
HRRM1 [STSG-10564]
規格:
價格:
編號:B224156
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
HRRM1 [STSG-10564]
商品貨號
B224156
Designations
HRRM1 [STSG-10564]
GenBank Number

X59617

培養基
Species
Homo sapiens, human
Depositors
Y Yen
Vector
Construct size (kb): 6.329999923706055
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pCRII
Intact vector size: 3.900
Type of vector: phagemid
Vector end: TA site
Vector end: TA site
Cloning sites: TA cloning between EcoRI sites
Polylinker sites: NsiI HindIII KpnI SacI BamHI SpeI BstXI EcoRI EcoRI EcoRV
BstXI NotI AvaI XmaIII XhoI NsiI XbaI ApaI
Host range: Escherichia coli
Features (with orientation and position when available):
insert detection: lacZ'
marker(s): ampR, kanR
promoter: lac, SP6, T7
replicon: pMB1
Cross references:
Insert
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: RRM1
Genomic copy number: unique
Gene name: ribonucleotide reductase M1 polypeptide
Contains complete coding sequence?: Y
Chromosome: 11; Localization: 11 p15.5-p15.4
Type of DNA: cDNA
Insert 5' end: Modification: SacI site
Insert 3' end: Modification: PstI site
Insert size (kb): 2.379
Cross references: DNA Seq. Acc.: X59617
Nucleotides ~1~2379 of the insert correspond to
nucleotides 182-2560 of X59617.
Insert lengths(kb): 2.378999948501587
Gene product: ribonucleotide reductase M1 polypeptide(ribonucleoside diphosphate reductase, M1 subunit) [RRM1]
Alleles: A2, A2, A1
Insert Size (kb)
2.379
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--4.6, 1.7; SacI--4.0, 2.3; PstI--2.7, 2.4, 1.2; HindIII--3.7, 2.3; PstI/SacI--2.7, 2.4, 1.2.
The insert contains the following restriction sites (approximate kb from the 5' end): HindIII--0.07; EcoRI--0.39, 0.93, 1.69; BamHI--0.53, 2.37.
Constructed by PCR using primers generating a SacI site just upstream of the initation codon (5' GCTCGAGCTCATG 3') and a PstI site just after the termination codon (5' TGAG CTGCAGCTGC 3').
References

Pavloff N, et al. Sequence analysis of the large and small subunits of human ribonucleotide reductase. DNA Seq. 2: 227-234, 1992. PubMed: 1627826

Yun Yen, personal communication

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