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當(dāng)前位置: 首頁(yè) > ATCC代理 > DR-beta-A [E. coli HB101(pBR322(Pst)/HLA-DR-beta-A)] B224222
DR-beta-A [E. coli HB101(pBR322(Pst)/HLA-DR-beta-A)]
DR-beta-A [E. coli HB101(pBR322(Pst)/HLA-DR-beta-A)]
規(guī)格:
價(jià)格:
編號(hào):B224222
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱(chēng)
DR-beta-A [E. coli HB101(pBR322(Pst)/HLA-DR-beta-A)]
商品貨號(hào)
B224222
Designations
DR-beta-A [E. coli HB101(pBR322(Pst)/HLA-DR-beta-A)]
GenBank Number

A06800

培養(yǎng)基
Species
Homo sapiens, human
Depositors
Biogen N.V., JF Haley, Biogen N.V.
Vector
Construct size (kb): 5.525000095367432
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBR322
Intact vector size: 4.363
Type of vector: plasmid
Vector end: PstI Modification: dG tailed
Vector end: PstI Modification: dG tailed
Cloning sites: EcoRI ClaI HindIII EcoRV BamHI SphI SalI XmaIII NruI BspMI
BsmI StyI AvaI BalI BspMII PvuII Tth111I NdeI AflIII PpaI
PstI PvuI ScaI SspI AatII
Polylinker sites:
Construction: pBR313
Host range: Escherichia coli
Features (with orientation and position when available):
marker(s): tetR
replicon: pMB1
marker(s): ampR
Cross references: DNA Seq. Acc.: J01749
Insert
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: human
Gene symbol: HLA-DRB*
Gene name: major histocompatibility complex, class II, DR beta
(GB:A06800)
Contains complete coding sequence?: Y
Chromosome: 6; Localization: 6 p21.3
Tissue: cell line IBW9 (beta lymphoblasts)
Type of DNA: cDNA
Insert end: Modification: dC tailed
Insert end: Modification: dC tailed
Insert size (kb): 1.162
Cross references: DNA Seq. Acc.: A06800
Nucleotides 1-1162 of the insert correspond to
nucleotides 1-1162 of A06800.
Insert lengths(kb): 1.161999940872192
Tissue: cell line IBW9 (beta lymphoblasts)
Gene product: major histocompatibility complex, class II, DR beta (GB:A06800) [HLA-DRB*]
Insert Size (kb)
1.162
Media
ATCC® Medium 1273: LB medium (ATCC medium 1065) with 20 mcg/ml tetracycline
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): PstI--4.4, 0.5 and smaller fragments; SstI--5.6; StuI--5.6.
The insert contains the following restriction sites (approximate kb from the 5' end): SacI--0.10; ScaI--0.30; PstI--0.36, 0.49, 0.97; XmnI--0.61; HindIII--0.89.
References

Mach BF, et al. DNA sequences coding for the DR-beta-chain locus of the human lymphocyte antigen complex and polypeptides, diagnostic typing processes and products related thereto. US Patent 5,169,941 dated Dec 8 1992

Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
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