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當(dāng)前位置: 首頁(yè) > ATCC代理 > 16C8m [pET-3cT] B224503
16C8m [pET-3cT]
16C8m [pET-3cT]
規(guī)格:
價(jià)格:
編號(hào):B224503
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
16C8m [pET-3cT]
商品貨號(hào)
B224503
Designations
16C8m [pET-3cT]
Species
Mus musculus, mouse
培養(yǎng)基
Depositors
ET Cocuzzi, SE Walther
Applications
in another host, produces protein tissue inhibitor of metalloproteinase 1 (erythroid potentiating activity, collagenase inhibitor)
Vector
Construct size (kb): 5.599999904632568
Insert
DNA: cDNA
Insert lengths(kb): 0.6000000238418579
Tissue: embryo fibroblasts
Gene product: tissue inhibitor of metalloproteinase 1 (erythroid potentiating activity, collagenase inhibitor) [Timp]
Target Gene: tissue inhibitor of metalloproteinase 1 (erythroid potentiating activity, collagenase inhibitor)
Insert Size (kb)
0.600
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): BamHI/NdeI--5.0, 0.6; PstI--3.9, 1.7; EcoRV--4.8, 0.55, 0.1; BamHI--5.6; MboII--1.65, 0.9, 0.75, 0.7, 0.6, 0.27, 0.1, +smaller.
The insert contains the following restriction sites (bp from the 5' end): HaeI--4; EcoRV--177; PstI--304; XmnI--251.
Constructed by modifying codon 23 from AAG to ATG, changing a StuI site to a NdeI site. The insert extends 49 bp 3' of the termination codon to the BamHI site.
Classification
Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod
References

Cocuzzi ET, et al. Expression and purification of mouse TIMP-1 from E. coli. FEBS Lett. 307: 375-378, 1992. PubMed: 1322839

Enzo T Cocuzzi, personal communication

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