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pG165/T1
pG165/T1
規格:
價格:
編號:B224588
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
pG165/T1
商品貨號
B224588
Designations
pG165/T1
GenBank Number

J05463

培養基
Species
Saccharomyces cerevisiae Meyen ex E.C. Hansen
Depositors
A Tzagoloff
Vector
Construct size (kb): 15.80000019073486
Insert
DNA: genomic
Insert lengths(kb): 8.0
Gene product: mitochondrial membrane protein, F1-F0 assembly [ATP10]
Insert Size (kb)
8.0
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments
The insert contains the following sites in kb from the vector SmaI site (or the published sequence): SstI--0.2 (+666), HindIII--0.6 (+289), EcoRI--0.7 (+197), BglII--2.2 (-1443), BglII--2.8, SphI--3.8, EcoRI--5.7, SphI--6.0, SstI (7.1).
Restriction digests of the clone give the following sizes (kb): SmaI/BglII--13.0, 2.8, 0.8; SstI--9.4, 8.7; BglII--16.0, 0.8; EcoRI--6.5 (doublet), 2.3 (doublet).
The ATP10 gene product is 30.3 kDa and appears to be required for the assembly of the F1-F0 ATPase. atp10 mutations decrease the rutamycin sensitivity of the ATPase.
The ATP10 sequences are contained in a 2.2 kb SmaI/BglII fragment which contains some sequences from the vector. This fragment also contains 2 other open reading frames.
Classification
Saccharomycetes, Saccharomycetidae, Saccharomycetales, Saccharomycetaceae, Saccharomycetaceae, Saccharomyces, cerevisiae
References

Ackerman SH, Tzagoloff A. ATP10, a yeast nuclear gene required for the assembly of the mitochondrial F1-F0 complex. J. Biol. Chem. 265: 9952-9959, 1990. PubMed: 2141026

Tanaka T. Restriction of plasmid-mediated transformation in Bacillus subtils 168. Mol. Gen. Genet. 175: 235-237, 1979. PubMed: 117281

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