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Avian reovirus type 3
Avian reovirus type 3
規格:
貨期:
編號:B224632
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
Avian reovirus type 3
商品貨號
B224632
Classification
Reoviridae, Reovirus
Agent
Avian reovirus type 3
Strain
59
Biosafety Level
2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Isolation
Intestine from chicken with cloacal pasting
Product Format
frozen
Storage Conditions
?-70°C or colder
Comments
Deposited as Avian reovirus type 3. Pathogenic for newborn mice.
Effect on Host
CPE, syncytia formation
?CPE, syncytia formation
Recommended Host
Primary chicken embryo fibroblasts

Production Host: ?Primary CEF

Growth Conditions
Temperature: 37°C
Recommendations for Infection: Plate cells 24-48 hours prior to infection. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.1). Adsorb 1-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
Incubation: 2 to 7 days in a humidified 5% CO2 atmosphere
Name of Depositor
BL Patel, University of Minnesota, College of Veterinary Medicine
Source
Intestine from chicken with cloacal pasting
References

Deshmukh DR, Pomeroy BS. Avian reoviruses. I. Isolation and serological characterization. Avian Dis. 13: 239-243, 1969. PubMed: 4307737

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