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D2a
D2a
規格:
價格:
編號:B224645
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
D2a
商品貨號
B224645
Designations
D2a
GenBank Number

L10717

培養基
Species
Homo sapiens, human
Depositors
GB Mills, S Gibson
Applications
A 2.8 kb PstI fragment from the unique and untranslated 5' domain has been used as a probe.
Vector
Construct size (kb): 12.39999961853027
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript KS
Intact vector size: 2.960
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: SacI BstXI SacII NotI XbaI SpeI BamHI SmaI PstI EcoRI EcoRV
HindIII ClaI (SalI HincII AccI) XhoI DraII ApaI KpnI
Polylinker sites: SEE COMMENTS
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
enhancer: none
insert detection: lacZ'
marker(s): ampR
promoter: lac, T3, T7
replicon: pMB1, f1
terminator: none
Cross references:
Insert
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: ITK
Genomic copy number: unique
Gene name: T-cell-specific tyrosine kinase; homolog of mouse T-cell
itk/tsk tyrosine kinase
Contains complete coding sequence?: Y
Chromosome: 5; Localization: 5 q31-q32
Tissue: thymus
Type of DNA: cDNA
Insert end: EcoRI
Insert end: EcoRI
Insert size (kb): 9.4
Cross references: DNA Seq. Acc.: L10717
Insert lengths(kb): 9.399999618530273
Tissue: thymus
Gene product: T-cell-specific tyrosine kinase; homolog of mouse T-cell itk/tsk tyrosine kinase [ITK]
Insert Size (kb)
9.400
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--6.5, 3.6, 2.5; EcoRI--4.8, 3.1, 2.5, 2.0; PstI--9.2, 2.8, 0.56; XbaI--5.9, 3.8, 1.8, 0.7; HindIII--4.5, 4.3, 2.5, 1.1.
The insert contains the following restriction sites (approximate kb from the 5' end): BamHI--5.92; BglI--2.13; BglII--4.67; EcoRI--1.95; EcoRV--4.48; HindIII--1.32, 2.43; KpnI--1.90; PstI--2.83, 3.39; SstI--2.19, 3.11; XbaI--5.26, 5.95.
Contains the complete coding sequence (nt 2024-3886).
A 2.8 kb PstI fragment from the unique and untranslated 5' domain has been used as a probe.
References

Gibson S, et al. Identification, cloning, and characterization of a novel human T-cell-specific tyrosine kinase located at the hematopoietin complex on chromosome 5q. Blood 82: 1561-1572, 1993. PubMed: 8364206

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