黄色小说视频-日本少妇高潮抽搐-国内自拍av-天堂中文资源在线-蜜桃视频在线入口www-91久久综合-亚洲视频在线一区-日日夜夜拍-国产精品资源在线观看-欧美激情国产精品免费-人妻少妇久久中文字幕-成人免费无码大片a毛片-香蕉污视频在线观看-日本电影成人-国内精品久久99人妻无码

歷史搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購物車 1 種商品 - 共0元
當前位置: 首頁 > ATCC代理 > pCMV8 B224695
pCMV8
pCMV8
規格:
價格:
編號:B224695
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
pCMV8
商品貨號
B224695
Designations
pCMV8
Depositors
DL Davis
培養基
Biosafety Level
1
Vector Information
Size (kb): 4.9120001792907720
DESCRIPTION OF VECTOR:
Intact vector size: 4.912
Type of vector: phagemid
Cloning sites: PstI SalI XbaI BamHI SmaI
Polylinker sites: PstI SalI XbaI BamHI SmaI
Construction: pTZ18R, bacteriophage f1, CMV promoter
Features (with orientation and position when available):
MCS: PstI...SmaI
transcription terminator: hGH
enhancer: SV40
marker(s): ampR, <-
replicon: f1, <-
promoter: CMV, ->
Vector: pCMV8 (phagemid)
Promoters: Promoter CMV
Construction: pTZ18R, bacteriophage f1, CMV promoter
Marker(s):ampR
Construct size (kb): 4.912000179290772
Features: marker(s): ampR
promoter: CMV
replicon: f1
MCS: PstI...SmaI
transcription terminator: hGH
enhancer: SV40
Applications
cloning vector
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--4.9; SalI--4.9; XbaI--4.9.
Should be grown in media containing ampicillin at a final concentration of 1 mg/ml to improve plasmid yields.
May be used in essentially all cultured cells in transient transfection assays, to establish permanent cell lines and to construct transgenic mice.
The pCMV7 plasmid contains a hybrid intron, while the pCMV8 plasmid contains both a hybrid intron and a 5' untranslated segment corresponding to the adenovirus major late tripartite leader sequence.
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Andersson S, et al. Cloning, structure, and expression of the mitochondrial cytochrome P-450 sterol 26-hydroxylase, a bile acid biosynthetic enzyme. J. Biol. Chem. 264: 8222-8229, 1989. PubMed: 2722778

Shipped
frozen
Shipping Information
Distributed: freeze-dried
  • 王經理
    手機號:17280875617
    QQ:1438578920
  • 胡經理
    手機號:13345964880
    QQ:2438244627
  • 周經理
    手機號:17757487661
    QQ:1296385441
  • 于經理
    手機號:18067160830
    QQ:2088210172
  • 沈經理
    手機號:19548299266
    QQ:2662369050
  • 李經理
    手機號:13626845108
    QQ:972239479