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alpha13 pcDNAI
alpha13 pcDNAI
規(guī)格:
價(jià)格:
編號(hào):B224777
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
alpha13 pcDNAI
商品貨號(hào)
B224777
Designations
alpha13 pcDNAI
Species
Mus musculus, mouse
培養(yǎng)基
Depositors
HR Bourne
Applications
contains sequence G protein, alpha subunit
Vector
Construct size (kb): 6.5
Insert
DNA: cDNA
Insert lengths(kb): 2.5
Gene product: G protein, alpha subunit
Target Gene: G protein, alpha subunit
Insert Size (kb)
2.5
Media
ATCC® Medium 1951: LB medium (ATCC medium 1065) with 50 mcg/ml ampicillin and 15 mcg/ml tetracycline
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--5.0, 0.87, 0.6; HindIII/XbaI--4.0, 2.0, 0.46; Xho--6.5.
Expresses protein in mammalian cells. XhoI site in vector polylinker was lost during cloning, but there are two internal XhoI sites in insert. T7 promoter is the closest to 5' end.
Alpha13 excised from pMOB and subcloned into pcDNAI using EcoRI and NsiI sites.
Classification
Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod
References

Voyno-Yasenetskaya T, et al. G alpha 13 stimulates Na-H exchange. J. Biol. Chem. 269: 4721-4724, 1994. PubMed: 8106440

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