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pQF60
pQF60
規格:
價格:
編號:B224828
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
pQF60
商品貨號
B224828
Designations
pQF60
Depositors
M Farinha
培養基
Biosafety Level
1
Host
Distribution host: Escherichia coli JM109
Vector Information
Size (kb): 5.8000001907348630
DESCRIPTION OF VECTOR:
Intact vector size: 5.800
Type of vector: plasmid
Cloning sites: SstI KpnI SmaI BamHI XbaI AatII mLuI NcoI BglII XhoI StuI
SphI HindIII
Polylinker sites: EcoRI SstI KpnI SmaI BamHI XbaI SalI AatII mLuI NcoI BglII
XhoI StuI PstI SphI HIndIII
Construction: pCB267, pQF40
Host range: broad host range; Escherichia coli
Features (with orientation and position when available):
insert detection: phoA
marker(s): ampR
promoter: none
replicon: pMB1, pRO1614 (1600)
terminator: T1
Vector: pQF60 (plasmid)
Promoters: Promoter none
Construction: pCB267, pQF40
Marker(s):ampR
Construct size (kb): 5.800000190734863
Features: insert detection: phoA
marker(s): ampR
promoter: none
replicon: pMB1, pRO1614 (1600)
terminator: T1
Applications
promoter-cloning vector
shuttle vector
Comments
Restriction digests of the clone give the following sizes (kb): HindIII--5.8; BamHI--5.8; EcoRI--4.6, 1.2; XbaI--5.8.
Promoter-cloning shuttle vector permitting visual detection of activity.
Uses the promoterless alkaline phosphatase gene (phoA) as the reporter group.
Translational stop codons in all reading frames are located between the MCS and the translational initiation codon of the reporter gene.
The order of the major features in this plasmid is: ori(pMB1) - ori(1600) - ampR - terminator - multiple cloning site - phoA.
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Farinha MA, Kropinski AM. Construction of broad-host-range plasmid vectors for easy visible selection and analysis of promoters. J. Bacteriol. 172: 3496-3499, 1990. PubMed: 2111810

Shipped
frozen
Shipping Information
Distributed: freeze-dried
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