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pAIM-NEO
pAIM-NEO
規格:
價格:
編號:B224877
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
pAIM-NEO
商品貨號
B224877
Designations
pAIM-NEO
GenBank Number

Z22576

培養基
Species
Homo sapiens, human
Depositors
F Sanchez-Madrid
Applications
in another host, produces protein CD69 antigen (p60, early T-cell activation antigen)
Vector
Construct size (kb): 7.699999809265137
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pcDNA I/Neo
Intact vector size: 7.000
Type of vector: phagemid
Vector end: BstXI
Vector end: BstXI
Cloning sites: HindIII BamHI BstXI EcoRV NotI XhoI NsiI XbaI
Polylinker sites: HindIII BamHI BstXI EcoRV BstXI NotI XhoI NsiI XbaI
Host range: Escherichia coli
Features (with orientation and position when available):
marker(s): supF, G418R
promoter: CMV
replicon: SV40, polyoma, pMB1, M13
terminator: SV40 polyadenylation
Cross references:
Insert
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: CD69
Genomic copy number: unique
Gene name: CD69 antigen (p60, early T-cell activation antigen)
Contains complete coding sequence?: Y
Chromosome: 12; Localization: 12 p13-p12
Tissue: lymphocyte, activated
Type of DNA: cDNA
Insert end: BstXI
Insert end: BstXI
Insert size (kb): 0.693
Cross references: DNA Seq. Acc.: Z22576
Nucleotides 1-694 of the insert correspond to
nucleotides 12-705 of Z22576.
Insert lengths(kb): 0.6930000185966492
Tissue: lymphocyte, activated
Gene product: CD69 antigen (p60, early T-cell activation antigen) [CD69]
Target Gene: CD69 antigen (p60, early T-cell activation antigen)
Insert Size (kb)
0.693
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): HindIII/NotI--7.7; BstXI--7.0, 0.7; HindIII--7.7; NcoI--2.45, 2.1, 1.55, 0.85 (doublet); SstI(SacI)--7.7.
The insert contains the following restriction sites (approximate kb from the 5' end): SacI--0.07; NcoI--0.52; BstXI--0.52.
Contains the complete coding sequence (nt 82-681 of the sequence record).
Expresses the protein from the CMV promoter in mammalian cells.
References

Lopez-Cabrera M, et al. Molecular cloning, expression, and chromosomal localization of the human earliest lymphocyte activation antigen AIM/CD69, a new member of the C-type animal lectin superfamily of signal-transmitting receptors. J. Exp. Med. 178: 537-547, 1993. PubMed: 8340758

Francisco Sanchez-Madrid, personal communication

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