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pha2m1 [phigf4]
pha2m1 [phigf4]
規(guī)格:
價(jià)格:
編號(hào):B224884
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱(chēng)
pha2m1 [phigf4]
商品貨號(hào)
B224884
Designations
pha2m1 [phigf4]
Species
Homo sapiens, human
培養(yǎng)基
Depositors
GI Bell
Vector
Construct size (kb): 5.900000095367432
Insert
DNA: cDNA
Insert lengths(kb): 2.099999904632568
Tissue: adult liver
Gene product: alpha-2-macroglobulin [A2M]
Alleles: B2, C2, E1, A2, B1, B1, C1, D1, D2, E2, F1, F2, A1, B2, E1, E2
Insert Size (kb)
2.100
Media
ATCC® Medium 1273: LB medium (ATCC medium 1065) with 20 mcg/ml tetracycline
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): PstI--3.8, 1.4, 0.66; EcoRI--5.9; ClaI--4.4, 1.48; AvaI--3.3, 2.6; BamHI--5.9.
The insert encodes amino acids 809-1451 of the alpha-2-microglobulin gene.
Codominant segregation of the BglII polymorphism demonstrated in 2 families.
Codominant segregation of the PvuII polymorphism demonstrated in 5 families.
Codominant segregation of the HinfI polymorphism demonstrated in 5 families (35 individuals).
Codominant segregation of the PvuII polymorphism demonstrated in 2 families (20 individuals). HaeIII, HinfI and PuvII RFLPs are independent mutations.
Enzyme(s) not detecting polymorphism (in a panel of 5): BamHI, HincII, RsaI, HindIII, MspI, PstI.
Enzyme(s) not detecting polymorphism: AvaII, BamHI, EcoRV, StuI, AluI, NciI, MspI, PstI.
Enzyme(s) not detecting polymorphism: HindIII, KpnI, MspI, PstI, SacI, ScaI, XbaI, XmnI.
References

Bell GI, et al. Human alpha2-macroglobulin gene is located on chromosome 12. Somatic Cell Mol. Genet. 11: 285-289, 1985. PubMed: 2408344

Threadgill DW, et al. Syntenic conservation between humans and cattle: II. human chromosome 12. Genomics 8: 29-34, 1990. PubMed: 2081597

Scambler PJ, et al. Two RFLPs for human alpha-2 macroglobulin (A2M). Nucleic Acids Res. 13: 6787, 1985. PubMed: 2414729

Marynen P, et al. Three RFLP's associated with the human alpha2-macroglobulin gene (A2M). Nucleic Acids Res. 13: 8287, 1985. PubMed: 2415929

Barbara Weiffenbach, personal communication

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