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pHGTH4
pHGTH4
規(guī)格:
價格:
編號:B225159
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
pHGTH4
商品貨號
B225159
Designations
pHGTH4
Species
Homo sapiens, human
培養(yǎng)基
Depositors
JR Kelsoe
Vector
Construct size (kb): 5.300000190734863
Insert
DNA: genomic
Insert lengths(kb): 2.299999952316284
Tissue: lymphoblast GM5933 cell line
Gene product: tyrosine hydroxylase [TH]
Alleles: A1, A2
Insert Size (kb)
2.300
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--3.2, 2.4; BamHI/HindIII--3.2, 1.6, 0.8; EcoRI--3.8, 1.5; PstI--3.6, 0.68, 1.3; PvuI--4.0, 1.0.
PstI was homoallelic for A2 in 6 subjects from Amish pedigree 110 (IMR 884) in which two other chromosome 11 RFLPs were reported linked to manic-depressive illness.
Insert contains exon 10 through the 3' end of the gene, i.e. exons 10-13 including 3' untranslated sequence.
References

O'Malley KL, et al. Isolation and characterization of the human tyrosine hydroxylase gene: identification of 5' alternative splice sites responsible for multiple mRNAs. Biochemistry 26: 6910-6914, 1987. PubMed: 2892528

Kelsoe JR, et al. Human tyrosine hydroxylase (TH) genomic fragment (pHGTH4) identifies a PstI polymorphism. Nucleic Acids Res. 16: 7760, 1988. PubMed: 2901074

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