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human Id-1a [phId-1a]
human Id-1a [phId-1a]
規格:
價格:
編號:B225233
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
human Id-1a [phId-1a]
商品貨號
B225233
Designations
human Id-1a [phId-1a]
GenBank Number

S78986

培養基
Species
Homo sapiens, human
Depositors
MA Israel
Applications
contains sequence inhibitor of DNA binding 1, dominant negative helix-loop-helix protein Inhibitor of DNA binding 1, dominant negative, Id1
Vector
Construct size (kb): 3.900000095367432
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript SK
Intact vector size: 2.960
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV
EcoRI PstI SmaI BamHI SpeI XbaI NotI XmaIII SacII BstXI SacI
Polylinker sites: SEE COMMENTS
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
enhancer: none
insert detection: lacZ'
marker(s): ampR
promoter: lac, T3, T7
replicon: pMB1, f1
terminator: none
Cross references:
Insert
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: ID1
Gene name: inhibitor of DNA binding 1, dominant negative
helix-loop-helix protein
Contains complete coding sequence?: Y
Chromosome: 20; Localization: 20 q11
Tissue: U251 glioblastoma cell line
Type of DNA: cDNA
Insert end: EcoRI
Insert end: EcoRI
Insert size (kb): 0.958
Cross references: DNA Seq. Acc.: S78986
Nucleotides 1-530 of the insert correspond to
nucleotides 1-530 of S78986.
Insert lengths(kb): 0.9580000042915344
Tissue: U251 glioblastoma cell line
Gene product: inhibitor of DNA binding 1, dominant negative helix-loop-helix protein( Inhibitor of DNA binding 1, dominant negative, Id1) [ID1]
Target Gene: inhibitor of DNA binding 1, dominant negative helix-loop-helix protein
Insert Size (kb)
0.958
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 0.95; BglI--1.9, 1.3, 0.7; SmaI--3.5, 0.45; PvuI--2.3, 1.1, 0.5; PvuII--2.5, 1.05, 0.4.
Insert contains the following restriction sites (approximate kb from the 5' end): BglI--0.47, 0.48; PvuI--0.51, 0.64; PvuII--0.11; SmaI--0.41; SphI--0.49.
The clones human Id-1a (ATCC 99522/3) and human Id-1b (ATCC 99524/5) represent alternatively spliced products of the same gene.
References

Zhu W, et al. Id gene expression during development and molecular cloning of the human Id-1 gene. Brain Res. Mol. Brain Res. 30: 312-326, 1995. PubMed: 7637581

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