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Rickettsia raoultii
Rickettsia raoultii
規格:
貨期:
編號:B225239
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
Rickettsia raoultii
商品貨號
B225239
Classification
Rickettsiaceae, Rickettsieae
Agent
Rickettsia raoultii
Strain
DnS14
Applications
Vector borne research
Positive for Mycoplasma
Yes
Biosafety Level
3

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Isolation
Isolated from Dermacentor nutalli in Siberia, in 1994
Product Format
frozen
Storage Conditions
?Store at -60°C or colder
Comments
This item is a representative of a newly identified rickettsial species (Rickettsia raoultii), a gram-negative, obligately intracellular and non-motile bacterium.
It is genetically identical to the type strain for this species (Khabarovsk, isolated in 2005) at all loci sequenced. The preparation available through ATCC tested positive for mycoplasma contamination (M.orale) by PCR.
Requests for this agent require special permits and a signed statement assuming all risks and responsibility for laboratory handling.
Bacteria visible in cells following staining with Gimenez stain
Effect on Host
CPE in the form of cell rounding and sloughing of infected cells.
Growth Medium
ATCC® Medium 30-2003: Eagle's Minimum Essential Medium (EMEM)
Fetal Bovine Serum
Recommended Host
Vero cells (ATCC CRL-1587)
Growth Conditions
Recommendation for Infection: 5 days at 32°C and 5% CO2 in EMEM (ATCC 30-2003; with 2 mM L-glutamine) supplememted with 2% FBS (ATCC 30-2020), following centrifugation at 700 g for one hour at 22°C
Name of Depositor
Dr. P. -E. Fournier
Special Collection
ATCC
Year of Origin
1994
References

Mediannikov, O., et. al. 2008. Rickettsia raoultii sp. nov., a spotted fever group rickettsia associated with Dermacentor ticks in Europe and Russia. Int. J Syst. Evol. Microbiol. Jul;58 (Pt 7):1635-39. PubMed: 18599708

Marrero, M and Raoult D. Centrifugation-shell vial technique for rapid detection of Mediterranean spotted fever rickettsia in blood culture. Am J Trop Med Hyg. 1989 Feb;40(2):197-9. PubMed: 2645804

Rydkina, E. et. al., New Rickettsiae in ticks collected in territories of the former soviet union. Emerg. Infect. Dis. 1999. Nov-Dec;5(6):811-4. PubMed: 10603217

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