Comments
Restriction digests of the clone give the following sizes (kb): BamHI--5.0; EcoRI--5.0; PstI--4.5, 0.75.
Vector contains the following restriction sites (approximate kb from nt 1): BamHI--0.34; BglII--3.47; EcoRI--0.39; KpnI--0.36, 5.02; NcoI--0.33, 2.87; PstI--2.58, 3.25; PvuI--0.51; PvuII--0.48, 3.20, 3.56, 3.90, 3.99; SacI--0.38, 5.03; SalI--2.57.
Differs from pKSM714 (ATCC 87160) only in the orientation of the pSM102 fragment containing lacIq, kanR and the trpA terminator.
Expression vector.
