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D15
D15
規格:
價格:
編號:B225314
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
D15
商品貨號
B225314
Designations
D15
GenBank Number

L10820

培養基
Species
Homo sapiens, human
Vector
Construct size (kb): 4.349999904632568
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript SK-
Intact vector size: 2.960
Type of vector: phagemid
Vector end: EcoRI
Vector end: XhoI
Cloning sites: KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV
EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI
Polylinker sites: SacI BstXI SacII NotI EagI XbaI SpeI BamHI SmaI PstI EcoRI
EcoRV HindIII (ClaI Bsp106I) (HincII AccI SalI) XhoI
(Eco0109I DraII ApaI) KpnI
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: f1, ->
insert detection: lacZ', <-
primer site: M13-20, ->
promoter: T7, ->
MCS: KpnI...SacI, ->
promoter: T3, <-
promoter: lac, <-
primer site: M13 reverse, <-
replicon: pMB1
marker(s): ampR, <-
Cross references:
Insert
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: FPR1
Gene name: formyl peptide receptor 1
Contains complete coding sequence?: Y
Chromosome: 19; Localization: 19 q13.4
Tissue: HL-60 cell line
Disease: promyelocytic leukemia
Type of DNA: cDNA
Insert 5' end: Modification: EcoRI linker
Insert 3' end: Modification: XhoI linker
Insert size (kb): 1.35
Cross references: DNA Seq. Acc.: L10820
Nucleotides 1-1350 of the insert correspond to
nucleotides 525-552, 5370-6599 of L10820.
Insert lengths(kb): 1.350000023841858
Tissue: HL-60 cell line promyelocytic leukemia
Disease: promyelocytic leukemia
Gene product: formyl peptide receptor 1 [FPR1]
Insert Size (kb)
1.350
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--4.3; XhoI--4.3; EcoRI/XhoI--2.9, 1.4; BamHI--4.3; PstI--3.2, 1.1.
The insert includes the following restriction sites (approximate kb from the 5' end): NcoI--0.25; PvuII--1.0; PstI--1.14; SacI--1.18.
Lacks exon 2 in the 5' untranslated region that is present in some alternatively spliced transcripts. Begins within exon 1 and contains the complete exon 3.
References

Murphy PM, et al. Sequence and organization of the human N-formyl peptide receptor-encoding gene. Gene 133: 285-290, 1993. PubMed: 8224916

Murphy PM, McDermott D. Functional expression of the human formyl peptide receptor in Xenopus oocytes requires a complementary human factor. J. Biol. Chem. 266: 12560-12567, 1991. PubMed: 1712023

Murphy PM, et al. A structural homologue of the N-formyl peptide receptor. Characterization and chromosome mapping of a peptide chemoattractant receptor family. J. Biol. Chem. 267: 7637-7643, 1992. PubMed: 1373134

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