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pUH84

規格:

價格:

編號:B225359

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

pUH84

商品貨號

B225359

Designations

pUH84

Depositors

B Henrich

培養基

Biosafety Level

Vector Information

Size (kb): 3.19

Vector: pUH84 (plasmid)

Marker(s):ampR

Construct size (kb): 3.19

Features: insert detection: phiX174lysis
marker(s): ampR
replicon: pMB1
repressor gene: lacIq

Applications

vector permitting positive selection for inserts

Comments

The plasmid may be lost from transformants if stored as stabs or at -20C. For propagation and maintenance use strains with repressor-overproducing alleles of lacI; for cloning use strains that do not overproduce the lac repressor.

A positive selection plasmid vector.

Media

ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Growth Conditions

Temperature: 37.0°C

References

Henrich B, Plapp R. Use of the lysis gene of bacteriophage phiX174 for the construction of a positive selection vector. Gene 42: 345-349, 1986. PubMed: 2942443

Henrich B, et al. Lysis of Escherichia coli by induction of cloned phiX174 genes. Mol. Gen. Genet. 185: 493-497, 1982. PubMed: 6285147

Shipped

freeze-dried

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