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pUCM11
pUCM11
規(guī)格:
價(jià)格:
編號:B225500
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
pUCM11
商品貨號
B225500
Designations
pUCM11
GenBank Number

M14211

培養(yǎng)基
Species
Homo sapiens, human
Depositors
MW Hentze
Applications
produces protein ferritin, heavy polypeptide 1 Ferritin subunit H
Vector
Construct size (kb): 9.699999809265137
Insert
DNA: genomic
Insert lengths(kb): 7.199999809265137
Tissue: liver
Gene product: ferritin, heavy polypeptide 1(Ferritin subunit H) [FTH1]
Target Gene: ferritin, heavy polypeptide 1
Insert Size (kb)
7.200
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): HindIII--7.0, 2.7; BamHI--4.3, 2.8, 2.5; EcoRI--6.6, 3.3; PstI--4.0, 2.8, 1.3, 0.8, 0.7, 0.5; SalI--9.8.
The insert contains the entire coding sequence (4 exons) plus upstream and downstream sequences. The EcoRI fragment in the insert is smaller than the genomic sequence it detects, suggesting truncation during construction.
Only a partial sequence of promoter, intron I, all exons and introns II and III is available.
Insert is expressed in transformed murine B6 fibroblasts.
A 0.7 kb PstI fragment which is upstream of the TATA sequence is unique and was used to map the gene. It does not cross-hybridize with hamster or mouse DNA.
References

Hentze MW, et al. Cloning, characterization, expression, and chromosomal localization of a human ferritin heavy-chain gene. Proc. Natl. Acad. Sci. USA 83: 7226-7230, 1986. PubMed: 3020541

Hentze MW, et al. A cis-acting element is necessary and sufficient for translational regulation of human ferritin expression in response to iron. Proc. Natl. Acad. Sci. USA 84: 6730-6734, 1987. PubMed: 3477805

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