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CRIPTO cDNA
CRIPTO cDNA
規(guī)格:
價格:
編號:B225517
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產品名稱
CRIPTO cDNA
商品貨號
B225517
Designations
CRIPTO cDNA
Species
Homo sapiens, human
培養(yǎng)基
Depositors
MG Persico
Applications
When transcription is controlled by an RSV long terminal repeat, the cDNA clone induced foci of transformation in NIH3T3 cells.
A probe from the 5' 900 bp of this insert has been used to detect mRNA in mouse and human teratocarcinoma cells.
Vector
Construct size (kb): 4.699999809265137
Insert
DNA: cDNA
Insert lengths(kb): 2.0
Tissue: teratocarcinoma NT2D1 cell line
Gene product: teratocarcinoma-derived growth factor 2 (retroposin, possibly expressed)(CRIPTO-like, epidermal growth factor-related oncogene) [TDGF1]
Insert Size (kb)
2.0
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--2.7, 2.0; BamHI--4.7; HindIII--4.7; SalI--4.7; XbaI--4.7.
The 900 bp probe can be excised by an EcoRI/EcoRV double digest.
When transcription is controlled by an RSV long terminal repeat, the cDNA clone induced foci of transformation in NIH3T3 cells.
Contains a complete coding sequence and an AT-rich 3' non-coding sequence of about 1200 bp. The 3' region includes an inverted Alu repeat, a non-canonical polyadenylation signal, and the ATTT(A) sequence of short-lived RNAs.
A probe from the 5' 900 bp of this insert has been used to detect mRNA in mouse and human teratocarcinoma cells.
References

Ciccodicola A, et al. Molecular characterization of a gene of the 'EGF family' expressed in undifferentiated human NTERA2 teratocarcinoma cells. EMBO J. 8: 1987-1991, 1989. PubMed: 2792079

Dono R, et al. Isolation and characterization of the CRIPTO autosomal gene and its X-linked related sequence. Am. J. Hum. Genet. 49: 555-565, 1991. PubMed: 1882841

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