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當(dāng)前位置: 首頁 > ATCC代理 > HHCPQ06 B225649
HHCPQ06
HHCPQ06
規(guī)格:
價格:
編號:B225649
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
HHCPQ06
商品貨號
B225649
Designations
HHCPQ06
GenBank Number

M78198

培養(yǎng)基
Species
Homo sapiens, human
Applications
The predicted product size is (bp): 117. The observed product size(s) is(are) (bp): 117 (human).
Vector
Construct size (kb): 4.300000190734863
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript SK-
Intact vector size: 2.960
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV
EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI
Polylinker sites: SacI BstXI SacII NotI EagI XbaI SpeI BamHI SmaI PstI EcoRI
EcoRV HindIII (ClaI Bsp106I) (HincII AccI SalI) XhoI
(Eco0109I DraII ApaI) KpnI
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: f1, ->
insert detection: lacZ', <-
primer site: M13-20, ->
promoter: T7, ->
MCS: KpnI...SacI, ->
promoter: T3, <-
promoter: lac, <-
primer site: M13 reverse, <-
replicon: pMB1
marker(s): ampR, <-
Cross references:
Insert
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: D19S387E
Genomic copy number: unique
Gene name: DNA Segment, single copy, expressed
Contains complete coding sequence?: U
Sequence homology: Homo sapiens inositol 1,4,5-trisphosphate 3-kinase
Chromosome: 19; Localization: 19 q13.1-q13.2
Tissue: hippocampus, female, 2 years, oligo-dT and random primed
(Stratagene 936205), subtracted
Type of DNA: cDNA
Insert end: Modification: EcoRI linkers
Insert end: Modification: EcoRI linkers
Insert size (kb): 1.1
Cross references: DNA Seq. Acc.: M78198; EST: EST01791; Primer: M13 Forward;
THC id: THC98332
Insert lengths(kb): 1.100000023841858
Tissue: hippocampus, female, 2 years, oligo-dT and random primed (Stratagene 936205), subtracted
Gene product: inositol 1,4,5-trisphosphate 3-kinase [ITPK*]
Insert Size (kb)
1.100
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--3.2, 1.1; BamHI--4.3; PvuII--2.7, 1.1, 0.5.
EST01791 can be detected in genomic DNA using the following oligonucleotides (5'-3'): GTGGCACATGACACAATCTC and CAGGTTAATTGGATGGCG.
EST01791 matches PIR accession JN0129 with 68.18% identity and 80.29% similarity over the sequence range: 348-151.
Appears to be chimeric, with the chromosome 19-specific portion showing homology to ITP3.
This sequence record is for human inositol 1,4,5-trisphosphate 3-kinase.
The predicted product size is (bp): 117. The observed product size(s) is(are) (bp): 117 (human).
References

Korenberg JR, et al. Toward a cDNA map of the human genome. Genomics 29: 364-370, 1995. PubMed: 8666383

Adams MD, et al. Sequence identification of 2,375 human brain genes. Nature 355: 632-634, 1992. PubMed: 1538749

Maglott DR, et al259 human brain expressed sequence tags (ESTs): chromosome localization, subregional assignment, and sequence analysisIn: Maglott DR, et alIdentification of transcribed sequencesNew YorkPlenum Presspp. 273-288, 1994

Gregory G Lennon, personal communication

J C Venter, personal communication

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