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pRep(A) [pREP]

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價格:

編號:B225755

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

pRep(A) [pREP]

商品貨號

B225755

Designations

pRep(A) [pREP]

Depositors

H Iba

培養基

Biosafety Level

Vector Information

Size (kb): 9.1999998092651370

Vector: pRep(A) (plasmid)

Construction: RSV (5' LTR, gag, pol, 5' env), pBR322

Marker(s):ampR

Construct size (kb): 9.199999809265137

Features: marker(s): ampR
other: 5' LTR
replicon: pMB1
restriction site: SalI
coding sequence: 5' env
coding sequence: gag
coding sequence: pol(derived from the Bryan strain of RSV

Applications

vector for production of recombinant retrovirus

Comments

Restriction digests of the clone give the following sizes (kb): EcoRV - 9.4; NdeI - 9.4; SalI - 9.4.

The parental plasmid pSR-REP which encodes pol, derived from the Schmidt-Ruppin subgroup A strain of RSV, was described in Cell 34: 597-607, 1983.

Rous sarcoma virus (RSV) derived vector (5' LTR, gag, pol and 5' env), for ligation with avian retroviral expression vectors (ATCC 87699 - 87701) to create proviral DNA capable of producing replication competent recombinant virus.

The vector can be linearized with SalI for ligation to one of the RSV-derived expression vectors.

The pol sequence is derived from the Bryan strain of RSV and is more efficient in virus production after transfection.

Media

ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Growth Conditions

Temperature: 37.0°C

References

Murakami M, et al. High-level expression of exogenous genes by replication-competent retrovirus vectors with an internal ribosomal entry site. Gene 202: 23-29, 1997. PubMed: 9427541

Iba H, et al. Transforming potential and growth stimulating activity of the v-fos and c-fos genes carried by avian retrovirus vectors. Oncogene Res. 2: 121-133, 1988. PubMed: 2851123

Iba H, et al. Rous sarcoma virus variants that carry the cellular src gene instead of the viral src gene cannot transform chicken embryo fibroblasts. Proc. Natl. Acad. Sci. USA 81: 4424-4428, 1984. PubMed: 6087323