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cMCOB19 [RW-203]
cMCOB19 [RW-203]
規(guī)格:
價(jià)格:
編號:B226107
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
cMCOB19 [RW-203]
商品貨號
B226107
Designations
cMCOB19 [RW-203]
Species
Homo sapiens, human
培養(yǎng)基
Depositors
RL White, Y Nakamura
Applications
RFLPs observed under normal stringency (0.1X SSC).
Vector
Construct size (kb): 28.0
Insert
DNA: genomic
Insert lengths(kb): 20.0
Gene product: DNA Segment, single copy [D19S20]
Insert Size (kb)
20.0
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): HpaI--1.8, 1.2, 0.9 + smaller; HindIII--16.9, 3.6, 3.2, 2.2, 1.9; EcoRI--19.6, 7.5; XbaI--28; AccI--12.3, 7.2, 6.3, 4.4, 1.6.
Depositor has verified that our preparation detects the described RFLP.
IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture.
RFLPs observed under normal stringency (0.1X SSC).
References

Nakamura Y, et al. New approach for isolation of VNTR markers. Am. J. Hum. Genet. 43: 854-859, 1988. PubMed: 2904220

Yusuke Nakamura, personal communication

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