欧美日韩国产成人在线,色秀视频网,精品精品精品

EBOpLPP

規格:

價格:

編號:B226190

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

加入購物車

在線咨詢收藏

產品名稱

EBOpLPP

商品貨號

B226190

Designations

EBOpLPP

Depositors

RF Margolskee

培養基

Biosafety Level

Vector Information

Size (kb): 10.5719995498657200

Vector: EBOpLPP (plasmid)

Promoters: Promoter SV40 early

Construction: pcDV, pL, EBOpSV2neo

Marker(s):ampR,hygR

Construct size (kb): 10.57199954986572

Features: marker(s): ampR
marker(s): hygR
promoter: SV40 early
replicon: EBV oriP
replicon: pMB1
terminator: SV40
enhancer: SV40

Applications

expression vector

shuttle vector

Comments

Restriction digests of the clone give the following sizes (kb): HindIII/EcoRI--7.0, 3.5, 0.6; EcoRI--7.0, 4.0; SalI--10.6; XbaI--10.6.

This is a shuttle vector permitting expression in primate cell lines. It is useful for subcloning.

This was also constructed from the SfiI fragment of EBOpSV2neo (hygromycin phosphotransferase gene, oriP, and EBNA-1 gene).

This was constructed from the HindIII/EcoRI fragment of pcDV (pBR322 ori, beta-lactamase, SV40 late polyadenylation signal) and the HindIII/PstI fragment of pL (SV40 early promoter and SV40 late intervening sequence).

Media

ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Growth Conditions

Temperature: 37.0°C

References

Canfield V, et al. Ouabain-resistant mutants of the rat Na,K-ATPase alpha2 isoform identified by using an episomal expression vector. Mol. Cell. Biol. 10: 1367-1372, 1990. PubMed: 2157137

Spickofsky N, et al. Procedures for constructing cDNA expression libraries in Epstein-Barr virus shuttle vectors capable of stable episomal replication. DNA Protein Eng. Tech. 2: 14-18, 1990.

Shipped

freeze-dried

王經理

手機號：17280875617

QQ：1438578920
胡經理

手機號：13345964880

QQ：2438244627
周經理

手機號：17757487661

QQ：1296385441
于經理

手機號：18067160830

QQ：2088210172
沈經理

手機號：19548299266

QQ：2662369050
李經理

手機號：13626845108

QQ：972239479