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Rat coronavirus
Rat coronavirus
規(guī)格:
價(jià)格:
編號(hào):B226363
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
Rat coronavirus
商品貨號(hào)
B226363
Classification
Nidovirales, Coronaviridae, Coronavirus,Group 2
Deposited As
Rat Coronavirus
培養(yǎng)基
Agent
Rat coronavirus
Strain
8190
Common Name
Rat coronavirus
Biosafety Level
2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Isolation
Lungs of rat
Product Format
frozen
Storage Conditions
?Store at -70°C or colder
Comments
Antigenic cross reactivity with mouse coronavirus. Rats inoculated with this material (mouse brain stock) do not seroconvert to other agents.
VR-1410 and VR-635 are the same strain provided by different contributors.
Antigenic cross reactivity with mouse coronavirus. Rats inoculated with this material (mouse brain stock) do not seroconvert to other agents.
Effect on Host
Yes, in vitro effects: Causes large syncytia in L2 or PRK cells
Yes, in vivo effects: respiratory distress and death in suckling rats
Recommended Host
L2 cells (ATCC CCL-149)

Recommended Host: ?L2 (Percy) (ATCC? CCL-81?)

rat; mouse; L2 cells; primary rat kidney cells
Growth Conditions
Temperature: 37°C
Recommendations for Infection: Plant cells 24 hours in advance and infect when cultures are 80-90% confluent. Remove medium and rinse cell sheet with 1X DPBS. Remove 1X DPBS and then inoculate with a small volume (e.g. 1 mL per 25 cm2) of virus diluted to provide a MOI of about 0.01. Adsorb for 1 hour at 37°C in a humidified 5% CO2 atmosphere. End adsorption by adding virus growth medium.
Incubation: Incubate infected culture for 2 days at 37°C in a humidified 5% CO2 atmosphere, until CPE are well advance through 90% of the culture.
Name of Depositor
DJ Gaertner
Special Collection
ATCC
Source
Lungs of rat
References

Parker JC, et al. Rat coronavirus (RCV): a prevalent, naturally occurring pneumotropic virus of rats. Arch. Gesamte Virusforsch. 31: 293-302, 1970. PubMed: 4099196

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