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pRCN005 [pRC2]

規(guī)格:

價(jià)格:

編號:B226605

品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株

定量菌液

DNA

RNA

規(guī)格:

凍干粉

斜面

甘油

平板

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產(chǎn)品名稱

pRCN005 [pRC2]

商品貨號

B226605

Designations

pRCN005 [pRC2]

GenBank Number

X08021

培養(yǎng)基

Species

Red clover necrotic mosaic virus

Depositors

Z Xiong

Vector

Construct size (kb): 4.300000190734863

Insert

DNA: cDNA

Insert lengths(kb): 1.435999989509583

Gene product: 35 kDa protein

Insert Size (kb)

1.436

Media

ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments

The insert contains the following restriction sites (nucleotide positions according to the published sequence): BglII--911; XbaI--1029.

Restriction digests of the clone give the following sizes (kb): EcoRI--4.3; HindIII--4.3; PstI--2.7, 1.5; consistent with deposited DNA.

This clone includes the final nucleotide positions 15-1451. The single large open reading frame extends from 80 to 1031. There are other in frame AUG codons at positions 309, 312, 377, 428, and 688.

The insert is oriented such that linearizing with EcoRI and transcription with SP6 RNA polymerase gives the positive polarity (sense) transcript whereas linearizing with HindIII and transcription with T7 RNA polymerase gives the antisense strand.

Classification

Dianthovirus

References

Lommel SA, et al. The nucleotide sequence and gene organization of red clover necrotic mosaic virus RNA-2. Nucleic Acids Res. 16: 8587-8602, 1988. PubMed: 3047682

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